[HTML][HTML] AMP-activated protein kinase α2 activity is not essential for contraction-and hyperosmolarity-induced glucose transport in skeletal muscle
N Fujii, MF Hirshman, EM Kane, RC Ho… - Journal of Biological …, 2005 - ASBMB
To examine the role of AMP-activated protein kinase (AMPK) in muscle glucose transport,
we generated muscle-specific transgenic mice (TG) carrying cDNAs of inactive α2 (α2i TG)
and α1 (α1i TG) catalytic subunits. Extensor digitorum longus (EDL) muscles from wild type
and TG mice were isolated and subjected to a series of in vitro incubation experiments. In
α2i TG mice basal α2 activity was barely detectable, whereas basal α1 activity was only
partially reduced. Known AMPK stimuli including 5-aminoimidazole-4-carboxamide-1-β-4 …
we generated muscle-specific transgenic mice (TG) carrying cDNAs of inactive α2 (α2i TG)
and α1 (α1i TG) catalytic subunits. Extensor digitorum longus (EDL) muscles from wild type
and TG mice were isolated and subjected to a series of in vitro incubation experiments. In
α2i TG mice basal α2 activity was barely detectable, whereas basal α1 activity was only
partially reduced. Known AMPK stimuli including 5-aminoimidazole-4-carboxamide-1-β-4 …