Muscle-derived hematopoietic stem cells are hematopoietic in origin

SL McKinney-Freeman, KA Jackson… - Proceedings of the …, 2002 - National Acad Sciences
SL McKinney-Freeman, KA Jackson, FD Camargo, G Ferrari, F Mavilio, MA Goodell
Proceedings of the National Academy of Sciences, 2002National Acad Sciences
It has recently been shown that mononuclear cells from murine skeletal muscle contain the
potential to repopulate all major peripheral blood lineages in lethally irradiated mice, but the
origin of this activity is unknown. We have fractionated muscle cells on the basis of
hematopoietic markers to show that the active population exclusively expresses the
hematopoietic stem cell antigens Sca-1 and CD45. Muscle cells obtained from 6-to 8-week-
old C57BL/6-CD45. 1 mice and enriched for cells expressing Sca-1 and CD45 were able to …
It has recently been shown that mononuclear cells from murine skeletal muscle contain the potential to repopulate all major peripheral blood lineages in lethally irradiated mice, but the origin of this activity is unknown. We have fractionated muscle cells on the basis of hematopoietic markers to show that the active population exclusively expresses the hematopoietic stem cell antigens Sca-1 and CD45. Muscle cells obtained from 6- to 8-week-old C57BL/6-CD45.1 mice and enriched for cells expressing Sca-1 and CD45 were able to generate hematopoietic but not myogenic colonies in vitro and repopulated multiple hematopoietic lineages of lethally irradiated C57BL/6-CD45.2 mice. These data show that muscle-derived hematopoietic stem cells are likely derived from the hematopoietic system and are a result not of transdifferentiation of myogenic stem cells but instead of the presence of substantial numbers of hematopoietic stem cells in the muscle. Although CD45-negative cells were highly myogenic in vitro and in vivo, CD45-positive muscle-derived cells displayed only very limited myogenic activity and only in vivo.
National Acad Sciences