Isolation and characterization of intermediate filaments
P Steinert, R Zackroff, M Aynardi-Whitman… - Methods in cell …, 1982 - Elsevier
Publisher Summary This chapter describes procedures developed for the isolation and
characterization of intermediate filaments (IFs) and their subunits from different types of
tissues and cells grown in culture. IFs represent one of the three major cytoskeletal
components of many eukaryote cells. They appear tubular in cross section and are about 10
nm in diameter. For this reason, they are also commonly referred to as “10-nm” or “100-A o”
filaments. Their structure is unaffected by metal ions, high energy phosphates, or reductions …
characterization of intermediate filaments (IFs) and their subunits from different types of
tissues and cells grown in culture. IFs represent one of the three major cytoskeletal
components of many eukaryote cells. They appear tubular in cross section and are about 10
nm in diameter. For this reason, they are also commonly referred to as “10-nm” or “100-A o”
filaments. Their structure is unaffected by metal ions, high energy phosphates, or reductions …