Characterization of an HLA-DQ2-specific monoclonal antibody: Influence of amino acid substitutions in DQβ 1∗ 0202
HD Viken, G Paulsen, LM Sollid, KEA Lundin… - Human immunology, 1995 - Elsevier
The HLA-DQ (α1∗ 0501, βl∗ 0201) and-DQ (αl∗ 0501, βl∗ 0202)(ie, DQ2) heterodimers are
probably involved in the pathogenesis of celiac disease and several other HLA-DQ-
associated diseases. To obtain a tool for studies of these molecules, a mAb of the IgGl
isotype, 2.12. E11, was produced by immunization with purified DQ (αl∗ 0501, βl∗ 0201)
molecules and murine NIH 3T3 cells transfected with both DQA1∗ 05011 and DQB 1∗
0202. Panel cell studies with HLA homozygous B-lymphoblastoid cells and HLA-transfected …
probably involved in the pathogenesis of celiac disease and several other HLA-DQ-
associated diseases. To obtain a tool for studies of these molecules, a mAb of the IgGl
isotype, 2.12. E11, was produced by immunization with purified DQ (αl∗ 0501, βl∗ 0201)
molecules and murine NIH 3T3 cells transfected with both DQA1∗ 05011 and DQB 1∗
0202. Panel cell studies with HLA homozygous B-lymphoblastoid cells and HLA-transfected …