[HTML][HTML] Mitochondria shape hormonally induced cytoplasmic calcium oscillations and modulate exocytosis
EJ Kaftan, T Xu, RF Abercrombie, B Hille - Journal of Biological Chemistry, 2000 - ASBMB
Pituitary gonadotropes transduce hormonal input into cytoplasmic calcium ([Ca 2+] cyt)
oscillations that drive rhythmic exocytosis of gonadotropins. Using Calcium Green-1 and
rhod-2 as optical measures of cytoplasmic and mitochondrial free Ca 2+, we show that
mitochondria sequester Ca 2+ and tune the frequency of [Ca 2+] cyt oscillations in rat
gonadotropes. Mitochondria accumulated Ca 2+ rapidly and in phase with elevations of [Ca
2+] cyt after GnRH stimulation or membrane depolarization. Inhibiting mitochondrial Ca 2+ …
oscillations that drive rhythmic exocytosis of gonadotropins. Using Calcium Green-1 and
rhod-2 as optical measures of cytoplasmic and mitochondrial free Ca 2+, we show that
mitochondria sequester Ca 2+ and tune the frequency of [Ca 2+] cyt oscillations in rat
gonadotropes. Mitochondria accumulated Ca 2+ rapidly and in phase with elevations of [Ca
2+] cyt after GnRH stimulation or membrane depolarization. Inhibiting mitochondrial Ca 2+ …