Biochemical evidence for functional heterogeneity of cardiac sarcoplasmic reticulum vesicles.
LR Jones, SE Cala - Journal of Biological Chemistry, 1981 - ASBMB
Two subpopulations of cardiac sarcoplasmic reticulum vesicles were resolved functionally,
based on their sensitivities to the drug ryanodine. These two subpopulations of sarcoplasmic
reticulum vesicles, termed ryanodine-sensitive and ryanodine-insensitive, were separated
by preloading crude cardiac microsomes with Ca2+ oxalate in the presence of ATP, followed
by sucrose density gradient centrifugation. Ryanodine-insensitive vesicles accumulated
most of the Ca2+ oxalate during the preload, and constituted the densest subfraction …
based on their sensitivities to the drug ryanodine. These two subpopulations of sarcoplasmic
reticulum vesicles, termed ryanodine-sensitive and ryanodine-insensitive, were separated
by preloading crude cardiac microsomes with Ca2+ oxalate in the presence of ATP, followed
by sucrose density gradient centrifugation. Ryanodine-insensitive vesicles accumulated
most of the Ca2+ oxalate during the preload, and constituted the densest subfraction …