[HTML][HTML] Cardiac-specific overexpression of mouse cardiac calsequestrin is associated with depressed cardiovascular function and hypertrophy in transgenic mice
Y Sato, DG Ferguson, H Sako, GW Dorn… - Journal of Biological …, 1998 - ASBMB
Calsequestrin is a high capacity Ca 2+-binding protein in the sarcoplasmic reticulum (SR)
lumen. To elucidate the functional role of calsequestrin in vivo, transgenic mice were
generated that overexpressed mouse cardiac calsequestrin in the heart. Overexpression (20-
fold) of calsequestrin was associated with cardiac hypertrophy and induction of a fetal gene
expression program. Isolated transgenic cardiomyocytes exhibited diminished shortening
fraction (46%), shortening rate (60%), and relengthening rate (60%). The Ca 2+ transient …
lumen. To elucidate the functional role of calsequestrin in vivo, transgenic mice were
generated that overexpressed mouse cardiac calsequestrin in the heart. Overexpression (20-
fold) of calsequestrin was associated with cardiac hypertrophy and induction of a fetal gene
expression program. Isolated transgenic cardiomyocytes exhibited diminished shortening
fraction (46%), shortening rate (60%), and relengthening rate (60%). The Ca 2+ transient …