12-O-tetradecanoyl-phorbol-13-acetate induction of the human collagenase gene is mediated by an inducible enhancer element located in the 5′-flanking region

P Angel, I Baumann, B Stein, H Delius… - … and Cellular Biology, 1987 - Am Soc Microbiol
P Angel, I Baumann, B Stein, H Delius, HJ Rahmsdorf, P Herrlich
Molecular and Cellular Biology, 1987Am Soc Microbiol
Genomic clones coding for human fibroblast collagenase were isolated. By constructing and
transfecting mutants with 5′ and 3′ deletion mutations of the 5′ control region of the
gene into human or murine cells, we delimited a 32-base-pair sequence at positions-73 to-
42 which is required for the induction of transcription by the tumor promoter 12-O-
tetradecanoyl-phorbol-13-acetate. The DNA element behaves as a 12-O-tetradecanoyl-
phorbol-13-acetate-inducible enhancer: it mediates the stimulation of transcription to the …
Abstract
Genomic clones coding for human fibroblast collagenase were isolated. By constructing and transfecting mutants with 5′ and 3′ deletion mutations of the 5′ control region of the gene into human or murine cells, we delimited a 32-base-pair sequence at positions-73 to-42 which is required for the induction of transcription by the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate. The DNA element behaves as a 12-O-tetradecanoyl-phorbol-13-acetate-inducible enhancer: it mediates the stimulation of transcription to the heterologous herpes simplex virus thymidine kinase promoter and acts in a position-and orientation-independent manner. Differences in enhancer efficiency in different cell lines are interpreted to indicate differences in the activity of a trans-acting factor.
American Society for Microbiology