[HTML][HTML] Homodimerization of soluble guanylyl cyclase subunits: Dimerization analysis using a glutathiones-transferase affinity tag
U Zabel, C Häusler, M Weeger… - Journal of Biological …, 1999 - ASBMB
Soluble guanylyl cyclase (sGC) is an α/β-heterodimeric hemoprotein that, upon interaction
with the intercellular messenger molecule NO, generates cGMP. Although the related family
of particulate guanylyl cyclases (pGCs) forms active homodimeric complexes, it is not known
whether homodimerization of sGC subunits occurs. We report here the expression in Sf9
cells of glutathione S-transferase-tagged recombinant human sGCα1 and β1 subunits,
applying a novel and rapid purification method based on GSH-Sepharose affinity …
with the intercellular messenger molecule NO, generates cGMP. Although the related family
of particulate guanylyl cyclases (pGCs) forms active homodimeric complexes, it is not known
whether homodimerization of sGC subunits occurs. We report here the expression in Sf9
cells of glutathione S-transferase-tagged recombinant human sGCα1 and β1 subunits,
applying a novel and rapid purification method based on GSH-Sepharose affinity …