Effects of methylene blue and LY83583 on neuronal nitric oxide synthase and NADPH-diaphorase
D Luo, S Das, SR Vincent - European Journal of Pharmacology: Molecular …, 1995 - Elsevier
Methylene blue and 6-anilino-5, 8-quinolinedine (LY83583) have often been used as
'selective'inhibitors of soluble guanylyl cyclase. We report that in vitro assays, both these
compounds were potent inhibitors of rat cerebellar nitric oxide synthase activity. Methylene
blue had an apparent Ki of 2.7 μM, while for LY83583 the Ki was 15.8 μM. Furthermore,
methylene blue, but not LY83583, inhibited the NADPH-diaphorase histochemical reaction
associated with nitric oxide synthase. Our results indicate that many of the effects of these …
'selective'inhibitors of soluble guanylyl cyclase. We report that in vitro assays, both these
compounds were potent inhibitors of rat cerebellar nitric oxide synthase activity. Methylene
blue had an apparent Ki of 2.7 μM, while for LY83583 the Ki was 15.8 μM. Furthermore,
methylene blue, but not LY83583, inhibited the NADPH-diaphorase histochemical reaction
associated with nitric oxide synthase. Our results indicate that many of the effects of these …