[HTML][HTML] Structural examination of autoregulation of multifunctional calcium/calmodulin-dependent protein kinase II
E Yang, H Schulman - Journal of Biological Chemistry, 1999 - ASBMB
Regulation of Ca 2+/calmodulin-dependent protein kinase II is likely based on an auto-
inhibitory mechanism in which a segment of the kinase occupies the catalytic site in the
absence of calmodulin. We analyze potential auto-inhibitory associations by employing
charge reversal and hydrophobic-to-charged residue mutagenesis. We identify interacting
amino acid pairs by using double mutants to test which modification in the catalytic domain
complements a given change in the auto-inhibitory domain. Our studies identify the core …
inhibitory mechanism in which a segment of the kinase occupies the catalytic site in the
absence of calmodulin. We analyze potential auto-inhibitory associations by employing
charge reversal and hydrophobic-to-charged residue mutagenesis. We identify interacting
amino acid pairs by using double mutants to test which modification in the catalytic domain
complements a given change in the auto-inhibitory domain. Our studies identify the core …