[HTML][HTML] Contrasting effects of IRS-1 versus IRS-2 gene disruption on carbohydrate and lipid metabolism in vivo
To examine the impact of homozygous genetic disruption of insulin receptor substrate (IRS)-
1 (IRS-1−/−) or IRS-2 (IRS-2−/−) on basal and insulin-stimulated carbohydrate and lipid
metabolism in vivo, we infused 18-h fasted mice (wild-type (WT), IRS-1−/−, and IRS-2−/−)
with [3-3 H] glucose and [2 H 5] glycerol and assessed rates of glucose and glycerol
turnover under basal (0–90 min) and hyperinsulinemic-euglycemic clamp (90–210 min; 5
mm glucose, and 5 milliunits of insulin· kg− 1· min− 1) conditions. Both IRS-1−/− and IRS …
1 (IRS-1−/−) or IRS-2 (IRS-2−/−) on basal and insulin-stimulated carbohydrate and lipid
metabolism in vivo, we infused 18-h fasted mice (wild-type (WT), IRS-1−/−, and IRS-2−/−)
with [3-3 H] glucose and [2 H 5] glycerol and assessed rates of glucose and glycerol
turnover under basal (0–90 min) and hyperinsulinemic-euglycemic clamp (90–210 min; 5
mm glucose, and 5 milliunits of insulin· kg− 1· min− 1) conditions. Both IRS-1−/− and IRS …