[HTML][HTML] Characterization of the subunit structure of the catalytically active type I iodothyronine deiodinase
JL Leonard, TJ Visser, DM Leonard - Journal of Biological Chemistry, 2001 - ASBMB
Type I iodothyronine deiodinase is a∼ 50-kDa, integral membrane protein that catalyzes the
outer ring deiodination of thyroxine. Despite the identification and cloning of a 27-kDa
selenoprotein with the catalytic properties of the type I enzyme, the composition and the
physical nature of the active deiodinase are unknown. In this report, we use a molecular
approach to determine holoenzyme composition, the role of the membrane anchor on
enzyme assembly, and the contribution of individual 27-kDa subunits to catalysis …
outer ring deiodination of thyroxine. Despite the identification and cloning of a 27-kDa
selenoprotein with the catalytic properties of the type I enzyme, the composition and the
physical nature of the active deiodinase are unknown. In this report, we use a molecular
approach to determine holoenzyme composition, the role of the membrane anchor on
enzyme assembly, and the contribution of individual 27-kDa subunits to catalysis …