In vitro differentiation of satellite cells isolated from normal and dystrophic mammalian muscles. A comparison with embryonic myogenic cells

G Cossu, B Zani, M Coletta, M Bouchè, M Pacifici… - Cell differentiation, 1980 - Elsevier
Satellite cells were isolated from skeletal muscles of adult normal and dystrophic mice
(C57/6J/dy strain) by sequential digestion of tissue fragments with collagenase,
hyaluronidase and trypsin. These cells exhibit in culture similar behaviour to that of
embryonic myoblasts, undergoing an initial duplicative period lasting about 2–3 days,
followed by a shorter phase (1–2 days) of rapid cell fusion. During the duplicative phase
most of the satellite cells appear round-shaped, whereas embryonic myoblasts appear …