[PDF][PDF] Metabolism of isolated fat cells: I. Effects of hormones on glucose metabolism and lipolysis

M Rodbell - Journal of Biological Chemistry, 1964 - worthington-biochem.com
M Rodbell
Journal of Biological Chemistry, 1964worthington-biochem.com
Increased attention has been focused on the metabolism of adipose tissue and its marked
sensitivity to various hormones (for reviews, see Vaughan (1) and Jeanrenaud (2)). In an
attempt to obtain a homogeneous preparation of fat cells, it was found that if rat adipose
tissue is treated with collagenase, fat cells are liberated. Owing to their high fat content, the
fat cells can be separated from t, he more dense stromal-vascular cells by flotat. ion.
Increased attention has been focused on the metabolism of adipose tissue and its marked sensitivity to various hormones (for reviews, see Vaughan (1) and Jeanrenaud (2)). In an attempt to obtain a homogeneous preparation of fat cells, it was found that if rat adipose tissue is treated with collagenase, fat cells are liberated. Owing to their high fat content, the fat cells can be separated from t, he more dense stromal-vascular cells by flotat. ion.
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