MuSK antibodies in AChR Ab-seropositive MG vs AChR Ab-seronegative MG

K Ohta, K Shigemoto, S Kubo, N Maruyama, Y Abe… - Neurology, 2004 - AAN Enterprises
K Ohta, K Shigemoto, S Kubo, N Maruyama, Y Abe, N Ueda, M Ohta
Neurology, 2004AAN Enterprises
Materials and methods. The fusion protein was constructed from the extracellular domains of
human MuSK and placental alkaline phosphatase expressed in secreted form from COS-7
cells. This rMuSK protein was purified in an alkaline phosphatase antibody-bound agarose
column. MuSK antibodies were detected with RIA. In brief, 5 μL of serum samples was
incubated with [125 I] rMuSK (40,000 cpm) overnight at 4 C. Then 50 μL of antihuman IgG
was added and incubated for 1 hour at room temperature. After two washes with saline …
Materials and methods.
The fusion protein was constructed from the extracellular domains of human MuSK and placental alkaline phosphatase expressed in secreted form from COS-7 cells. This rMuSK protein was purified in an alkaline phosphatase antibody-bound agarose column. MuSK antibodies were detected with RIA. In brief, 5 μL of serum samples was incubated with [125 I] rMuSK (40,000 cpm) overnight at 4 C. Then 50 μL of antihuman IgG was added and incubated for 1 hour at room temperature. After two washes with saline, radioactivity in the pellets was counted.
American Academy of Neurology