Acetycholine receptor antibody characteristics in myasthenia gravis: Fractionation of α-Bungarotoxin binding site antibodies and their relationship to IgG subclass
PJ Whiting, A Vincent, J Newsom-Davis - Journal of neuroimmunology, 1983 - Elsevier
Anti-acetylcholine receptor (anti-AChR) antibodies from two myasthenia gravis (MG)
patients, known to have anti-AChR to the α-bungarotoxin (α-BuTx) binding site on the AChR
(anti-α-BuTx site), were fractionated on an affinity column of human AChR fully saturated
with α-BuTx. More than 80% of the anti-AChR in the pass-through fractions was directed
against the α-BuTx site, whereas anti-AChR eluted from the column was mostly directed at
other site on the AChR. Recovery of anti-AChR was greater than 60%. Anti-α-BuTx site …
patients, known to have anti-AChR to the α-bungarotoxin (α-BuTx) binding site on the AChR
(anti-α-BuTx site), were fractionated on an affinity column of human AChR fully saturated
with α-BuTx. More than 80% of the anti-AChR in the pass-through fractions was directed
against the α-BuTx site, whereas anti-AChR eluted from the column was mostly directed at
other site on the AChR. Recovery of anti-AChR was greater than 60%. Anti-α-BuTx site …