Specific immunotherapy of experimental myasthenia gravis in vitro: the “guided missile” strategy
JM Wu, B Wu, A Miagkov, RN Adams, DB Drachman - Cellular Immunology, 2001 - Elsevier
We describe a strategy for specific immunotherapy of myasthenia gravis (MG) based on
genetic engineering of antigen presenting cells (APCs) to present the autoantigen
acetylcholine receptor (AChR) and express the “warhead” Fas ligand (FasL). For
transduction of APCs we prepared recombinant attenuated vaccinia virus vectors carrying
the following three gene constructs:(i) AChR fused to LAMP1 to present AChR and target
AChR-specific T cells;(ii) FasL to eliminate the targeted T cells; and (iii) truncated FADD to …
genetic engineering of antigen presenting cells (APCs) to present the autoantigen
acetylcholine receptor (AChR) and express the “warhead” Fas ligand (FasL). For
transduction of APCs we prepared recombinant attenuated vaccinia virus vectors carrying
the following three gene constructs:(i) AChR fused to LAMP1 to present AChR and target
AChR-specific T cells;(ii) FasL to eliminate the targeted T cells; and (iii) truncated FADD to …