Analysis of high-resolution two-dimensional electrophoresis of differentiation-dependent alterations in cytosolic protein pattern of HL-60 leukemic cells
PR Jungblut, R Seifert - Journal of biochemical and biophysical methods, 1990 - Elsevier
HL-60 leukemic cells were differentiated along the neutrophilic pathway with retinoic acid
(RA) or along the monocytic pathway with 1, 25-dihydroxyvitamin D 3 (1, 25 (OH) 2 D 3).
Using a high-resolution two-dimensional electrophoresis technique and subsequent silver
staining, differentiation-dependent changes in cytosolic protein pattern of HL-60 cells were
analysed and were compared with the cytosolic protein pattern of human neutrophils. The
amount of 64 and 50 out of a total of 632 proteins studied was increased or decreased in RA …
(RA) or along the monocytic pathway with 1, 25-dihydroxyvitamin D 3 (1, 25 (OH) 2 D 3).
Using a high-resolution two-dimensional electrophoresis technique and subsequent silver
staining, differentiation-dependent changes in cytosolic protein pattern of HL-60 cells were
analysed and were compared with the cytosolic protein pattern of human neutrophils. The
amount of 64 and 50 out of a total of 632 proteins studied was increased or decreased in RA …