[HTML][HTML] Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties
N Itano, T Sawai, M Yoshida, P Lenas… - Journal of Biological …, 1999 - ASBMB
Three mammalian hyaluronan synthase genes, HAS1, HAS2, and HAS3, have recently
been cloned. In this study, we characterized and compared the enzymatic properties of
these three HAS proteins. Expression of any of these genes in COS-1 cells or rat 3Y1
fibroblasts yielded de novo formation of a hyaluronan coat. The pericellular coats formed by
HAS1 transfectants were significantly smaller than those formed by HAS2 or HAS3
transfectants. Kinetic studies of these enzymes in the membrane fractions isolated from HAS …
been cloned. In this study, we characterized and compared the enzymatic properties of
these three HAS proteins. Expression of any of these genes in COS-1 cells or rat 3Y1
fibroblasts yielded de novo formation of a hyaluronan coat. The pericellular coats formed by
HAS1 transfectants were significantly smaller than those formed by HAS2 or HAS3
transfectants. Kinetic studies of these enzymes in the membrane fractions isolated from HAS …