Expression of prostanoid receptors in human ductus arteriosus
A Leonhardt, A Glaser, M Wegmann… - British journal of …, 2003 - Wiley Online Library
A Leonhardt, A Glaser, M Wegmann, D Schranz, H Seyberth, R Nüsing
British journal of pharmacology, 2003•Wiley Online LibraryProstaglandins play a major role in maintaining ductal patency in utero. Ductal tone is
regulated by both locally released and circulating vasodilatory prostaglandins. In infants with
ductus arteriosus‐dependent congenital heart disease, ductal patency is maintained by
intravenous administration of prostaglandin (PG) E1. Little information is available regarding
the expression of prostaglandin receptors in man. By means of RT–PCR and
immunohistochemistry we studied the expression of the PGI2 receptor (IP), the four different …
regulated by both locally released and circulating vasodilatory prostaglandins. In infants with
ductus arteriosus‐dependent congenital heart disease, ductal patency is maintained by
intravenous administration of prostaglandin (PG) E1. Little information is available regarding
the expression of prostaglandin receptors in man. By means of RT–PCR and
immunohistochemistry we studied the expression of the PGI2 receptor (IP), the four different …
- Prostaglandins play a major role in maintaining ductal patency in utero. Ductal tone is regulated by both locally released and circulating vasodilatory prostaglandins. In infants with ductus arteriosus‐dependent congenital heart disease, ductal patency is maintained by intravenous administration of prostaglandin (PG) E1. Little information is available regarding the expression of prostaglandin receptors in man.
- By means of RT–PCR and immunohistochemistry we studied the expression of the PGI2 receptor (IP), the four different PGE2 receptors (EP1, EP2, EP3 and EP4), and the receptors for thromboxane (Tx) A2 (TP), PGD2 (DP) and PGF2α (FP) in the ductus arteriosus of three newborn infants with ductus arteriosus‐dependent congenital heart disease and intravenous infusion of PGE1 and of one 8 month old child with a patent ductus arteriosus.
- The EP3, EP4, FP, IP and TP receptor were markedly expressed at the mRNA and protein level, whereas the EP2 receptor was weakly expressed and the EP1 receptor was detected in two out of four tissue specimens only. The DP receptor was not detected in any of the samples. The most pronounced expression, which was located in the media of the ductus arteriosus, was observed for the EP4 and TP receptors followed by IP and FP receptor protein.
- These data indicate that ductal patency during the infusion of PGE1 in infants with ductus arteriosus‐dependent congenital heart disease might be mediated by the EP4 and IP receptor. The data further suggest that a heterogeneous population of prostanoid receptors may contribute to the regulation of ductus arteriosus tone in humans.
British Journal of Pharmacology (2003) 138, 655–659. doi:10.1038/sj.bjp.0705092
Wiley Online Library