Transgenic engineering of male-specific muscular hypertrophy
D Pirottin, L Grobet, A Adamantidis… - Proceedings of the …, 2005 - National Acad Sciences
Proceedings of the National Academy of Sciences, 2005•National Acad Sciences
Using a two-step procedure involving insertional gene targeting and recombinase-mediated
cassette exchange in ES cells, we have produced two lines of transgenic mice expressing a
dominant-negative latency-associated myostatin propeptide under control of the myosin light
chain 1F promoter and 1/3 enhancer from the TSPY locus on the Y chromosome. Males of
the corresponding lines are characterized by a 5-20% increase in skeletal muscle mass.
This experiment demonstrates the feasibility of a more efficient cattle production system …
cassette exchange in ES cells, we have produced two lines of transgenic mice expressing a
dominant-negative latency-associated myostatin propeptide under control of the myosin light
chain 1F promoter and 1/3 enhancer from the TSPY locus on the Y chromosome. Males of
the corresponding lines are characterized by a 5-20% increase in skeletal muscle mass.
This experiment demonstrates the feasibility of a more efficient cattle production system …
Using a two-step procedure involving insertional gene targeting and recombinase-mediated cassette exchange in ES cells, we have produced two lines of transgenic mice expressing a dominant-negative latency-associated myostatin propeptide under control of the myosin light chain 1F promoter and 1/3 enhancer from the TSPY locus on the Y chromosome. Males of the corresponding lines are characterized by a 5-20% increase in skeletal muscle mass. This experiment demonstrates the feasibility of a more efficient cattle production system combining superior beef production abilities for bulls and dairy abilities for cows.
National Acad Sciences