A rapid flow cytometric method for determining the cellular composition of bronchoalveolar lavage fluid cells in mouse models of asthma

LS van Rijt, H Kuipers, N Vos, D Hijdra… - Journal of …, 2004 - Elsevier
Mouse models of allergic asthma are increasingly used to study the immunopathology of this
complex disorder. The degree and type of airway inflammation is often studied by
determination of differential cell counts on cytospins of bronchoalveolar lavage fluid (BALF)
cells stained with May–Grünwald Giemsa, in which the separation of eosinophils (eos) from
neutrophils (neutro) and of monocytes (mono) from activated T cells can be quite
problematic. In this study, we compared differential cell counts based on morphological …