Elastase-like activity in cultured aortic endothelial cells

S Menashi, W Hornebeck, L Robert, Y Legrand - Thrombosis research, 1989 - Elsevier
Cultured porcine aortic endothelial cells were studied for cellular and secreted elastase
activity. We describe an activity hydrolyzing the synthetic elastase substrate, succinyl
(alanine) 3 nitroanilide, but not elastin, which was shown to be membrane located and was
not secreted to the culture medium. A different neutral proteinase activity degrading
insoluble elastin was demonstrated in the culture medium following its fractionation by gel
filtration high performance liquid chromatography (HPLC). Since no elastinolytic activity …