Quantitative evaluation of mammalian skeletal muscle as a heterologous protein expression system
M DiFranco, P Ñeco, J Capote, P Meera… - Protein expression and …, 2006 - Elsevier
The production of mammalian proteins in sufficient quantity and quality for structural and
functional studies is a major challenge in biology. Intrinsic limitations of yeast and bacterial
expression systems preclude their use for the synthesis of a significant number of
mammalian proteins. This creates the necessity of well-identified expression systems based
on mammalian cells. In this paper, we demonstrate that adult mammalian skeletal muscle,
transfected in vivo by electroporation with DNA plasmids, is an excellent heterologous …
functional studies is a major challenge in biology. Intrinsic limitations of yeast and bacterial
expression systems preclude their use for the synthesis of a significant number of
mammalian proteins. This creates the necessity of well-identified expression systems based
on mammalian cells. In this paper, we demonstrate that adult mammalian skeletal muscle,
transfected in vivo by electroporation with DNA plasmids, is an excellent heterologous …
