Expression and kinetics of cytokines determined by intracellular staining using flow cytometry
B Mascher, P Schlenke, M Seyfarth - Journal of immunological methods, 1999 - Elsevier
Cytokine production by human lymphocytes was assessed by a flow cytometric procedure
involving staining of intracellular cytokines by the paraformaldehyde-saponin procedure.
The production of interleukin 2 (IL2), interferon γ (IFNγ) and tumor necrosis factor α (TNFα)
was determined in T-helper (Th) and cytotoxic T-cells (CTL) as well as in naive and memory
cells after stimulation with phorbol-12-myristate-13-acetate (PMA) and ionomycin under the
influence of monensin. Kinetic studies on IL2, IFNγ and TNFα in lymphocyte subpopulations …
involving staining of intracellular cytokines by the paraformaldehyde-saponin procedure.
The production of interleukin 2 (IL2), interferon γ (IFNγ) and tumor necrosis factor α (TNFα)
was determined in T-helper (Th) and cytotoxic T-cells (CTL) as well as in naive and memory
cells after stimulation with phorbol-12-myristate-13-acetate (PMA) and ionomycin under the
influence of monensin. Kinetic studies on IL2, IFNγ and TNFα in lymphocyte subpopulations …