Yeast CAL1 is a structural and functional homologue to the DPR1 (RAM) gene involved in ras processing
A 2.3-kilobase pair DNA fragment of the yeast CAL1 gene was cloned by complementation
of the cal1-1 mutation, which causes a defect in nuclear division and bud formation (Ohya,
Y., Ohsumi, Y., and Anraku, Y.(1984) Mol. & Gen. Genet. 193, 389-394). Nucleotide
sequencing of this fragment revealed a single open reading frame (ORF) encoding a
polypeptide of 376 amino acids. Comparative analysis of the predicted amino acid
sequence has shown that the CAL1 product has similarity to two yeast proteins: the DPR1 …
of the cal1-1 mutation, which causes a defect in nuclear division and bud formation (Ohya,
Y., Ohsumi, Y., and Anraku, Y.(1984) Mol. & Gen. Genet. 193, 389-394). Nucleotide
sequencing of this fragment revealed a single open reading frame (ORF) encoding a
polypeptide of 376 amino acids. Comparative analysis of the predicted amino acid
sequence has shown that the CAL1 product has similarity to two yeast proteins: the DPR1 …