[HTML][HTML] Ruthenium red modifies the cardiac and skeletal muscle Ca2+ release channels (ryanodine receptors) by multiple mechanisms
L Xu, A Tripathy, DA Pasek, G Meissner - Journal of Biological Chemistry, 1999 - ASBMB
The effects of ruthenium red (RR) on the skeletal and cardiac muscle ryanodine receptors
(RyRs) were studied in vesicle-Ca 2+ flux,[3 H] ryanodine binding, and single channel
measurements. In vesicle-Ca 2+ flux measurements, RR was more effective in inhibiting
RyRs at 0.2 μm than 20 μm free Ca 2+.[3 H] Ryanodine binding measurements suggested
noncompetitive interactions between RR inhibition and Ca 2+ regulatory sites of RyRs. In
symmetric 0.25 m KCl with 10–20 μm cytosolic Ca 2+, cytosolic RR decreased single …
(RyRs) were studied in vesicle-Ca 2+ flux,[3 H] ryanodine binding, and single channel
measurements. In vesicle-Ca 2+ flux measurements, RR was more effective in inhibiting
RyRs at 0.2 μm than 20 μm free Ca 2+.[3 H] Ryanodine binding measurements suggested
noncompetitive interactions between RR inhibition and Ca 2+ regulatory sites of RyRs. In
symmetric 0.25 m KCl with 10–20 μm cytosolic Ca 2+, cytosolic RR decreased single …