[PDF][PDF] The in vivo distribution of autologous human and murine lymphoid cells grown in T cell growth factor (TCGF): implications for the adoptive immunotherapy of …
MT Lotze, BR Line, DJ Mathisen, SA Rosenberg - J Immunol, 1980 - scholar.archive.org
MT Lotze, BR Line, DJ Mathisen, SA Rosenberg
J Immunol, 1980•scholar.archive.orgMATERIALS AND METHODS Preparation of human lymphoid cells. Three humans with
pulmonary metastases from sarcomas that had proven resistant to standard chemotherapy
treatment were studied after injection of autologous lymphoid cells expanded in TCGF. The
procedures to be employed were carefully explained to the patients, and signed informed
consents were obtained from them. Venous blood from each individual was applied to fresh
Ficoll-Hypaque gradients to separate mononuclear cells as previously described (4). These …
pulmonary metastases from sarcomas that had proven resistant to standard chemotherapy
treatment were studied after injection of autologous lymphoid cells expanded in TCGF. The
procedures to be employed were carefully explained to the patients, and signed informed
consents were obtained from them. Venous blood from each individual was applied to fresh
Ficoll-Hypaque gradients to separate mononuclear cells as previously described (4). These …
MATERIALS AND METHODS
Preparation of human lymphoid cells. Three humans with pulmonary metastases from sarcomas that had proven resistant to standard chemotherapy treatment were studied after injection of autologous lymphoid cells expanded in TCGF. The procedures to be employed were carefully explained to the patients, and signed informed consents were obtained from them. Venous blood from each individual was applied to fresh Ficoll-Hypaque gradients to separate mononuclear cells as previously described (4). These cells were then expanded in 2-ml wells of 24-well Costar cluster plates (Costar, Cambridge, MA)
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