Localization of sialic acid in kidney glomeruli: regionalization in the podocyte plasma membrane and loss in experimental nephrosis.

PM Charest, J Roth - … of the National Academy of Sciences, 1985 - National Acad Sciences
PM Charest, J Roth
Proceedings of the National Academy of Sciences, 1985National Acad Sciences
Sialic acid residues were localized by electron microscopy in renal glomeruli of normal and
puromycin-treated rats with a cytochemical technique that utilized the Limax flavus lectin. In
Lowicryl K4M thin sections from normal rats, sialic acid residues were found along the
plasma membrane of the various glomerular cell types and in the glomerular basement
membrane as well as the mesangial matrix. In NaDodSO4/PAGE, sialic acid residues of
normal glomeruli were mainly confined to a 140-kDa protein previously identified as …
Sialic acid residues were localized by electron microscopy in renal glomeruli of normal and puromycin-treated rats with a cytochemical technique that utilized the Limax flavus lectin. In Lowicryl K4M thin sections from normal rats, sialic acid residues were found along the plasma membrane of the various glomerular cell types and in the glomerular basement membrane as well as the mesangial matrix. In NaDodSO4/PAGE, sialic acid residues of normal glomeruli were mainly confined to a 140-kDa protein previously identified as podocalyxin. The distribution of sialic acid residues in the podocyte plasma membrane was found to be remarkably regionalized. Based on the differential labeling intensity, three plasma membrane domains could be defined: the foot process base, the foot process region above the slit diaphragm, and the body of podocytes. Cytochemical and biochemical analysis of glomeruli from puromycin-treated rats showed a loss of sialic acid residues from glomerular sialoglycoconjugates indicating a perturbated glycosylation.
National Acad Sciences