[HTML][HTML] Mitochondrial glycerol phosphate acyltransferase directs the incorporation of exogenous fatty acids into triacylglycerol
RA Igal, S Wang, M Gonzalez-Baró… - Journal of Biological …, 2001 - ASBMB
The mitochondrial isoform of glycerol-3-phosphate acyltransferase (GPAT), the first step in
glycerolipid synthesis, is up-regulated by insulin and by high carbohydrate feeding via
SREBP-1c, suggesting that it plays a role in triacylglycerol synthesis. To test this hypothesis,
we overexpressed mitochondrial GPAT in Chinese hamster ovary (CHO) cells. When GPAT
was overexpressed 3.8-fold, triacylglycerol mass was 2.7-fold higher than in control cells.
After incubation with trace [14 C] oleate (∼ 3 μm), control cells incorporated 4.7-fold more …
glycerolipid synthesis, is up-regulated by insulin and by high carbohydrate feeding via
SREBP-1c, suggesting that it plays a role in triacylglycerol synthesis. To test this hypothesis,
we overexpressed mitochondrial GPAT in Chinese hamster ovary (CHO) cells. When GPAT
was overexpressed 3.8-fold, triacylglycerol mass was 2.7-fold higher than in control cells.
After incubation with trace [14 C] oleate (∼ 3 μm), control cells incorporated 4.7-fold more …