Deletion of a DNA polymerase β gene segment in T cells using cell type-specific gene targeting
H Gu, JD Marth, PC Orban, H Mossmann, K Rajewsky - Science, 1994 - science.org
H Gu, JD Marth, PC Orban, H Mossmann, K Rajewsky
Science, 1994•science.orgDeletion of the promoter and the first exon of the DNA polymerase β gene (pol β) in the
mouse germ line results in a lethal phenotype. With the use of the bacteriophage-derived,
site-specific recombinase Cre in a transgenic approach, the same mutation can be
selectively introduced into a particular cellular compartment-in this case, T cells. The impact
of the mutation on those cells can then be analyzed because the mutant animals are viable.
mouse germ line results in a lethal phenotype. With the use of the bacteriophage-derived,
site-specific recombinase Cre in a transgenic approach, the same mutation can be
selectively introduced into a particular cellular compartment-in this case, T cells. The impact
of the mutation on those cells can then be analyzed because the mutant animals are viable.
Deletion of the promoter and the first exon of the DNA polymerase β gene (pol β) in the mouse germ line results in a lethal phenotype. With the use of the bacteriophage-derived, site-specific recombinase Cre in a transgenic approach, the same mutation can be selectively introduced into a particular cellular compartment-in this case, T cells. The impact of the mutation on those cells can then be analyzed because the mutant animals are viable.
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