A method for the isolation, fluorescent labeling, and reinfusion of rat neutrophils for in vivo
investigation of white blood cell function in the microcirculation is described. The cell surface
morphology and function of the labeled and unlabeled neutrophils were compared in vitro
and in vivo. The morphology of the labeled and unlabeled cells was visually assessed by
differential interference contrast microscopy before and after exposure to the chemotactic
peptide FMLP. Labeled and unlabeled cells were morphologically similar under normal and …