Identification of the HLA-DRB1∗ 04,-DRB1∗ 07, and-DRB1∗ 09 Alleles by PCR Amplification with sequence-specific primers (PCR-SSP) in 2 hours
H Zetterquist, O Olerup - Human immunology, 1992 - Elsevier
The clinical applicability of genomic HLA class II typing techniques has increased after the
introduction of PCR-based typing strategies. In typing by PCR amplification using sequence
using sequence-specific primers (PCR-SSP), amplification of specific alleles or groups of
alleles is achieved, provided that the mismatch (es) of the SSP is located in the 3′ end of
the primer. Thus, the specificity of the typing system becomes part of the amplification step,
which reduces the total typing time to a minimum by simplifying the postamplification …
introduction of PCR-based typing strategies. In typing by PCR amplification using sequence
using sequence-specific primers (PCR-SSP), amplification of specific alleles or groups of
alleles is achieved, provided that the mismatch (es) of the SSP is located in the 3′ end of
the primer. Thus, the specificity of the typing system becomes part of the amplification step,
which reduces the total typing time to a minimum by simplifying the postamplification …