Robert W. Mahley,* Karl H. Weisgraber, Thomas Innerarity, H. Bryan Brewer, Jr., and Gerd Assmann abstract: Cholesterol feeding in miniature swine resulted in a hypercholesterolemia with a distinctive hyperlipoproteinemia and the subsequent development of atherosclero-sis. Alterations in the type and distribution of plasma lipoproteins induced by cholesterol feeding were as follows:(a) the occurrence of/3-migrating lipoproteins (B-VLDL) as well as very low density lipoproteins in the d< 1.006 ultracentrifugal fraction;(b) an increased prominence of the intermediate lipoproteins (d=

1.006-1.02);(c) an increased prominence of low density lipoproteins; and (d) the occurrence of a distinctive lipoprotein with a mobility which was referred to as HDLC (cholesterol induced). Characterization of the various plasma lipoproteins included chemical composition, size by electron microscopy, and apoprotein content. The B-VLDL resembled the/3-migrating lipopro-