[HTML][HTML] Endothelial nitric-oxide synthase (type III) is activated and becomes calcium independent upon phosphorylation by cyclic nucleotide-dependent protein …
E Butt, M Bernhardt, A Smolenski, P Kotsonis… - Journal of Biological …, 2000 - ASBMB
Endothelial nitric-oxide synthase (NOS-III) is defined as being strictly dependent on Ca
2+/calmodulin (CaM) for activity, although NO release from endothelial cells has been
reported to also occur at intracellular free Ca 2+ levels that are substimulatory for the purified
enzyme. We demonstrate here that NOS-III, but neither NOS-I nor-II, is rapidly and strongly
activated and phosphorylated on both Ser and Thr in the presence of cGMP-dependent
protein kinase II (cGK II) and the catalytic subunit of cAMP-dependent protein kinase (cAK) in …
2+/calmodulin (CaM) for activity, although NO release from endothelial cells has been
reported to also occur at intracellular free Ca 2+ levels that are substimulatory for the purified
enzyme. We demonstrate here that NOS-III, but neither NOS-I nor-II, is rapidly and strongly
activated and phosphorylated on both Ser and Thr in the presence of cGMP-dependent
protein kinase II (cGK II) and the catalytic subunit of cAMP-dependent protein kinase (cAK) in …