Expression, purification, and functional assays for self-association of dynamin-1
H Damke, AB Muhlberg, S Sever, S Sholly… - Methods in …, 2001 - Elsevier
Dynamin is a 100-kDa GTPase required for receptor-mediated endocytosis. It is specifically
targeted to clathrin-coated pits where it self-assembles into helical rings and is believed to
be required for late stages of clathrin-coated vesicle formation. 1, 2, 3 Self-assembly of
dynamin tetramers into higher order structures stimulates its GTPase activity, 4, 5 indicating
that dynamin-dynamin interactions are critical for regulating its cellular function. Dynamin
has been shown to contain six distinct domains through both sequence homology and …
targeted to clathrin-coated pits where it self-assembles into helical rings and is believed to
be required for late stages of clathrin-coated vesicle formation. 1, 2, 3 Self-assembly of
dynamin tetramers into higher order structures stimulates its GTPase activity, 4, 5 indicating
that dynamin-dynamin interactions are critical for regulating its cellular function. Dynamin
has been shown to contain six distinct domains through both sequence homology and …