High affinity binding sites for epidermal growth factor (EGF) in renal membranes
E Sack, Z Talor - Biochemical and biophysical research communications, 1988 - Elsevier
E Sack, Z Talor
Biochemical and biophysical research communications, 1988•ElsevierThe kidney produces large quantities of EGF but the role of the kidney in binding and
degradation of EGF is unknown. We studied 125 I-EGF binding and degradation by highly
purified cortical luminal and cortical basolateral membranes of rabbit renal cortex, and by
medullary plasma membranes. Specific binding for 125 I-EGF was found for the first time in
cortical basolateral and medullary plasma membranes (60–80% of total binding) but not in
cortical luminal membranes. There was little degradation (less than 4%) of EGF by any of the …
degradation of EGF is unknown. We studied 125 I-EGF binding and degradation by highly
purified cortical luminal and cortical basolateral membranes of rabbit renal cortex, and by
medullary plasma membranes. Specific binding for 125 I-EGF was found for the first time in
cortical basolateral and medullary plasma membranes (60–80% of total binding) but not in
cortical luminal membranes. There was little degradation (less than 4%) of EGF by any of the …
Abstract
The kidney produces large quantities of EGF but the role of the kidney in binding and degradation of EGF is unknown. We studied 125I-EGF binding and degradation by highly purified cortical luminal and cortical basolateral membranes of rabbit renal cortex, and by medullary plasma membranes. Specific binding for 125I-EGF was found for the first time in cortical basolateral and medullary plasma membranes (60–80% of total binding) but not in cortical luminal membranes. There was little degradation (less than 4%) of EGF by any of the membranes. Scatchard analysis of 125I-EGF binding by cortical basolateral membranes revealed two distinct classes of binding sites: high and low affinity. The existence of high specific binding sites in cortical basolateral and in medullary plasma membranes suggests a physiologic role of EGF in the kidney.
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