[HTML][HTML] HIV-1 integration in the human genome favors active genes and local hotspots

ARW Schröder, P Shinn, H Chen, C Berry, JR Ecker… - Cell, 2002 - cell.com
ARW Schröder, P Shinn, H Chen, C Berry, JR Ecker, F Bushman
Cell, 2002cell.com
A defining feature of HIV replication is integration of the proviral cDNA into human DNA. The
selection of chromosomal targets for integration is crucial for efficient viral replication, but the
mechanism is poorly understood. Here we describe mapping of 524 sites of HIV cDNA
integration on the human genome sequence. Genes were found to be strongly favored as
integration acceptor sites. Global analysis of cellular transcription indicated that active genes
were preferential integration targets, particularly genes that were activated in cells after …
Abstract
A defining feature of HIV replication is integration of the proviral cDNA into human DNA. The selection of chromosomal targets for integration is crucial for efficient viral replication, but the mechanism is poorly understood. Here we describe mapping of 524 sites of HIV cDNA integration on the human genome sequence. Genes were found to be strongly favored as integration acceptor sites. Global analysis of cellular transcription indicated that active genes were preferential integration targets, particularly genes that were activated in cells after infection by HIV-1. Regional hotspots for integration were also found, including a 2.4 kb region containing 1% of sites. These data document unexpectedly strong biases in integration site selection and suggest how selective targeting promotes aggressive HIV replication.
cell.com