Apoptosis of Vβ8. 2+ T lymphocytes in the spinal cord during recovery from experimental autoimmune encephalomyelitis induced in Lewis rats by inoculation with …

PA McCombe, I Nickson, Z Tabi, MP Pender - Journal of the neurological …, 1996 - Elsevier
To study T cell apoptosis during spontaneous recovery from experimental autoimmune
encephalomyelitis (EAE), we extracted lymphocytes from the spinal cords of Lewis rats with
EAE induced by inoculation with myelin basic protein (MBP) and adjuvants. Using flow
cytometry we assessed the numbers of CD5+ and TCRαβ+ lymphocytes, as well as Vβ8. 2+
lymphocytes, which constitute the predominant encephalitogenic MBP-reactive cells in
Lewis rats. Rats developed neurological signs of disease 10–12 days after inoculation. The …