Purification and characterization of the principal inhibitor of calcium oxalate monohydrate crystal growth in human urine.
Y Nakagawa, V Abram, FJ Kezdy, ET Kaiser… - Journal of Biological …, 1983 - ASBMB
Using an assay of the rate of crystal growth of calcium [14C] oxalate monohydrate, we
ascertained that the factor responsible for more than 90% of the crystal growth inhibition in
human urine is a nondialyzable macromolecule. We have purified this factor using DEAE-
cellulose chromatography, followed by Bio-Gel P-10 column chromatography with 50%
formamide as the eluent, and finally by gel permeation chromatography. About 5 mg of the
inhibitor was obtained from normal 24-h adult urine, with 16% recovery of the original …
ascertained that the factor responsible for more than 90% of the crystal growth inhibition in
human urine is a nondialyzable macromolecule. We have purified this factor using DEAE-
cellulose chromatography, followed by Bio-Gel P-10 column chromatography with 50%
formamide as the eluent, and finally by gel permeation chromatography. About 5 mg of the
inhibitor was obtained from normal 24-h adult urine, with 16% recovery of the original …