[HTML][HTML] Lateral clustering of platelet GP Ib-IX complexes leads to up-regulation of the adhesive function of integrin αIIbβ3
A Kasirer-Friede, J Ware, L Leng, P Marchese… - Journal of Biological …, 2002 - ASBMB
Binding of von Willebrand factor (VWF) to GP Ib-IX mediates initial platelet adhesion and
increases the subsequent adhesive function of α IIb β 3. Because these responses are
promoted most effectively by large VWF multimers, we hypothesized that receptor clustering
modulates GP Ib-IX function. To test this, GP IX was fused at its cytoplasmic tail to tandem
repeats of FKBP, and GP Ib-IX (FKBP) 2 and α IIb β 3 were expressed in Chinese hamster
ovary cells. Under flow conditions at wall shear rates of up to 2000 s− 1, GP Ib-IX (FKBP) 2 …
increases the subsequent adhesive function of α IIb β 3. Because these responses are
promoted most effectively by large VWF multimers, we hypothesized that receptor clustering
modulates GP Ib-IX function. To test this, GP IX was fused at its cytoplasmic tail to tandem
repeats of FKBP, and GP Ib-IX (FKBP) 2 and α IIb β 3 were expressed in Chinese hamster
ovary cells. Under flow conditions at wall shear rates of up to 2000 s− 1, GP Ib-IX (FKBP) 2 …