[HTML][HTML] Association of the membrane proximal regions of the α and β subunit cytoplasmic domains constrains an integrin in the inactive state
C Lu, J Takagi, TA Springer - Journal of Biological Chemistry, 2001 - ASBMB
The adhesiveness of integrins is regulated through a process termed" inside-out" signaling.
To understand the molecular mechanism of integrin inside-out signaling, we generated
K562 stable cell lines that expressed LFA-1 (α L β 2) or Mac-1 (α M β 2) with mutations in the
cytoplasmic domain. Complete truncation of the β 2 cytoplasmic domain, but not a truncation
that retained the membrane proximal eight residues, resulted in constitutive activation of α L
β 2 and α M β 2, demonstrating the importance of this membrane proximal region in the …
To understand the molecular mechanism of integrin inside-out signaling, we generated
K562 stable cell lines that expressed LFA-1 (α L β 2) or Mac-1 (α M β 2) with mutations in the
cytoplasmic domain. Complete truncation of the β 2 cytoplasmic domain, but not a truncation
that retained the membrane proximal eight residues, resulted in constitutive activation of α L
β 2 and α M β 2, demonstrating the importance of this membrane proximal region in the …