[HTML][HTML] Molecular Studies of the Asymmetric Pore Structure of the Human Cardiac Voltage-dependent Ca2+ Channel: CONSERVED RESIDUE, GLU-1086 …

U Klückner, G Mikala, A Schwartz, G Varadi - Journal of Biological …, 1996 - ASBMB
Proton transfer to calcium channels results in rapid fluctuations between two non-zero
conductance levels when the current is carried by monovalent cations. A combination of site-
directed mutagenesis and single-channel recording techniques were used to identify the
unique proton acceptor site as Glu-1086, a conserved glutamate residue located in the S5-
S6 linker of motif III of calcium channels. Glu-1086 is part of an array of four glutamate
residues in the pore-lining region of the channel conferring the high selectivity of calcium …