Replication-dependent potent IFN-α induction in human plasmacytoid dendritic cells by a single-stranded RNA virus

V Hornung, J Schlender, M Guenthner-Biller… - The Journal of …, 2004 - journals.aai.org
V Hornung, J Schlender, M Guenthner-Biller, S Rothenfusser, S Endres, KK Conzelmann
The Journal of Immunology, 2004journals.aai.org
Plasmacytoid dendritic cells sense viral ssRNA or its degradation products via TLR7/8 and
CpG motifs within viral DNA via TLR9. Although these two endosomal pathways operate
independently of viral replication, little is known about the detection of actively replicating
viruses in plasmacytoid dendritic cell (PDC). Replication and transcription of the viral
genome of ssRNA viruses as well as many DNA viruses lead to the formation of cytosolic
dsRNA absent in noninfected cells. In this study, we used human respiratory syncytial virus …
Abstract
Plasmacytoid dendritic cells sense viral ssRNA or its degradation products via TLR7/8 and CpG motifs within viral DNA via TLR9. Although these two endosomal pathways operate independently of viral replication, little is known about the detection of actively replicating viruses in plasmacytoid dendritic cell (PDC). Replication and transcription of the viral genome of ssRNA viruses as well as many DNA viruses lead to the formation of cytosolic dsRNA absent in noninfected cells. In this study, we used human respiratory syncytial virus (HRSV) encoding a fusion (F) protein for direct cytosolic entry. Both HRSV infection and cytosolic delivery of a 65-nt dsRNA led to potent IFN-α induction in PDC, but not in myeloid dendritic cells. Inactivation of HRSV by UV irradiation abrogated IFN-α induction in PDC. The comparison of two respiratory syncytial virus (RSV) constructs carrying either the HRSV or the bovine RSV F protein revealed that F-mediated cytosolic entry of RSV was absolutely required for IFN-α induction in PDC. HRSV-induced IFN-α production was independent of endosomal acidification and of protein kinase R (PKR) kinase activity, as demonstrated with chloroquine and the PKR inhibitor 2-aminopurine, respectively. In contrast, the induction of IFN-α by the TLR7/8 ligand R848, by the TLR9 ligand CpG-A ODN 2216, and by inactivated influenza virus (TLR7/8 dependent) was completely blocked by 2-aminopurine. IFN-α induction by mouse pathogenic Sendai virus was not affected in PKR-and MyD88-deficient mice, confirming that a ssRNA virus, which is able to directly enter host cells via fusion at the plasma membrane, can be detected by PDC independently of PKR, TLR7/8, and TLR9.
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