Direct transfer of p65 into T lymphocytes from systemic lupus erythematosus patients leads to increased levels of interleukin-2 promoter activity
TM Herndon, YT Juang, EE Solomou, SW Rothwell… - Clinical …, 2002 - Elsevier
The recent identification of a number of molecular defects in T cells from patients with
systemic lupus erythematosus (SLE) has raised expectations for gene replacement therapy
as an option in the treatment of these diseases. In this report, we have adapted an
electroporation-based technique to transfer successfully DNA to peripheral blood T cells
from normal individuals and patients with systemic lupus erythematosus and rheumatoid
arthritis. Transfection efficiency, judged by the percentage of live cells expressing green …
systemic lupus erythematosus (SLE) has raised expectations for gene replacement therapy
as an option in the treatment of these diseases. In this report, we have adapted an
electroporation-based technique to transfer successfully DNA to peripheral blood T cells
from normal individuals and patients with systemic lupus erythematosus and rheumatoid
arthritis. Transfection efficiency, judged by the percentage of live cells expressing green …