Production and effects of platelet-activating factor in the rat brain
R Kumar, SAK Harvey, M Kester, DJ Hanahan… - … et Biophysica Acta (BBA …, 1988 - Elsevier
The synthesis of platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-
phosphocholine) in rat brain was evaluated. Extracted PAF was characterized using
standard HPLC and TLC techniques, and by correlation of its bioactivity with the acetylation
state of the 2-position of the molecule. PAF was quantified by bioassay, its ability to cause| 3
H] serotonin release from washed rabbit platelets. The low basal level of PAF (0.25±0.15
pmol/g wet wt., mean±SE) in the brain of the intact rat was greatly increased by …
phosphocholine) in rat brain was evaluated. Extracted PAF was characterized using
standard HPLC and TLC techniques, and by correlation of its bioactivity with the acetylation
state of the 2-position of the molecule. PAF was quantified by bioassay, its ability to cause| 3
H] serotonin release from washed rabbit platelets. The low basal level of PAF (0.25±0.15
pmol/g wet wt., mean±SE) in the brain of the intact rat was greatly increased by …