Optimal conditions for the preparation of Fab and F (ab′) 2 fragments from monoclonal IgG of different rat IgG subclasses
J Rousseaux, R Rousseaux-Prevost, H Bazin - Journal of immunological …, 1983 - Elsevier
The optimal conditions for the preparation of Fab and F (ab′) 2 fragments from monoclonal
rat IgG of different subclasses are described. Digestion of IgG for 2–4 h at 37° C with
1%(w/w) papaïn at pH 7.0 in the presence of 0.01 M cysteine leads to almost complete
cleavage into Fab and Fc fragments. Fab fragments are isolated by sequential
chromatography on Ultrogel AcA 44 and on DEAE-cellulose columns. In the case of IgG2c
subclass, Fab fragment may be directly isolated by chromatography on Protein A …
rat IgG of different subclasses are described. Digestion of IgG for 2–4 h at 37° C with
1%(w/w) papaïn at pH 7.0 in the presence of 0.01 M cysteine leads to almost complete
cleavage into Fab and Fc fragments. Fab fragments are isolated by sequential
chromatography on Ultrogel AcA 44 and on DEAE-cellulose columns. In the case of IgG2c
subclass, Fab fragment may be directly isolated by chromatography on Protein A …