NF-AT activation induced by a CAML-interacting member of the tumor necrosis factor receptor superfamily

GU von Bulow, RJ Bram - Science, 1997 - science.org
GU von Bulow, RJ Bram
Science, 1997science.org
Activation of the nuclear factor of activated T cells transcription factor (NF-AT) is a key event
underlying lymphocyte action. The CAML (calcium-modulator and cyclophilin ligand) protein
is a coinducer of NF-AT activation when overexpressed in Jurkat T cells. A member of the
tumor necrosis factor receptor superfamily was isolated by virtue of its affinity for CAML.
Cross-linking of this lymphocyte-specific protein, designated TACI (transmembrane activator
and CAML-interactor), on the surface of transfected Jurkat cells with TACI-specific antibodies …
Activation of the nuclear factor of activated T cells transcription factor (NF-AT) is a key event underlying lymphocyte action. The CAML (calcium-modulator and cyclophilin ligand) protein is a coinducer of NF-AT activation when overexpressed in Jurkat T cells. A member of the tumor necrosis factor receptor superfamily was isolated by virtue of its affinity for CAML. Cross-linking of this lymphocyte-specific protein, designated TACI (transmembrane activator and CAML-interactor), on the surface of transfected Jurkat cells with TACI-specific antibodies led to activation of the transcription factors NF-AT, AP-1, and NFκB. TACI-induced activation of NF-AT was specifically blocked by a dominant-negative CAML mutant, thus implicating CAML as a signaling intermediate.
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