Expansion and functional activity of Ly‐1+ B cells upon transfer of peritoneal cells into allotype‐congenic, newborn mice

I Förster, K Rajewsky - European journal of immunology, 1987 - Wiley Online Library
I Förster, K Rajewsky
European journal of immunology, 1987Wiley Online Library
Transfer of peritoneal cells from adult donors into newborn, allotype‐congenic mice led to
colonization of the recipient mice by donor‐derived B lymphocytes expressing the Ly‐1
surface marker (Ly‐1 B cells). These cells not only persisted in the recipient mice for at least
5 months, but also increased in number. In contrast, bone marrow‐derived stem cells did not
or scarcely give rise to B cells after intraperitoneal injection into congenic newborn
recipients under the same experimental conditions. Approximately half of the IgM in the …
Abstract
Transfer of peritoneal cells from adult donors into newborn, allotype‐congenic mice led to colonization of the recipient mice by donor‐derived B lymphocytes expressing the Ly‐1 surface marker (Ly‐1 B cells). These cells not only persisted in the recipient mice for at least 5 months, but also increased in number. In contrast, bone marrow‐derived stem cells did not or scarcely give rise to B cells after intraperitoneal injection into congenic newborn recipients under the same experimental conditions.
Approximately half of the IgM in the serum of peritoneal cell‐recipients was produced by donor‐derived Ly‐1 B cells, suggesting that high levels of serum IgM in a normal mouse are produced by this B cell subpopulation. The transferred Ly‐1 B cells were able to respond in a normal fashion to α(1 → 3)dextran, but they did not participate in thymus‐dependent and ‐independent (TI II) immune responses to the hapten (4‐hydroxy‐3‐nitrophenyl)acetyl (NP). In neither the immune response to α(1 → 3)dextran nor that to NP were we able to detect an influence of the transferred Ly‐1 B cells on the selection of the idiotypic repertoire in the recipient mice.
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